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Hindawi Publishing Corporation | Evidence-Based Complementary and Alternative Medicine | Volume 2011, Article ID 286320, 12 pages
Modulation of Signal Proteins: A PlausibleMechanism to Explain How a Potentized Drug Secale Cor 30C Diluted beyond Avogadro's Limit Combats Skin Papilloma in Mice
Anisur Rahman Khuda-Bukhsh,1 Soumya Sundar Bhattacharyya,1 Saili Paul,1 Suman Dutta,1 Naoual Boujedaini,2 and Philippe Belon2
1Cytogenetics and Molecular Biology Laboratory, Department of Zoology, University of Kalyani, Kalyani 741235, West Bengal, India 2Boiron Laboratory, Lyon, France
Copyright © 2011 Anisur Rahman Khuda-Bukhsh et al. This is an open access article.
In homeopathy, ability of ultra-high diluted drugs at or above potency 12C (diluted beyond Avogadro's limit) in ameliorating/curing various diseases is often questioned, particularly because the mechanism of action is not precisely known. We tested the hypothesis if suitable modulations of signal proteins could be one of the possible pathways of action of a highly diluted homeopathic drug, Secale cornutum 30C (diluted 1060 times; Sec cor 30). It could successfully combat DMBA + croton oilinduced skin papilloma in mice as evidenced by histological, cytogenetical, immunofluorescence, ELISA and immunoblot findings. Critical analysis of several signal proteins like AhR, PCNA, Akt, Bcl-2, Bcl-xL, NF-κB and IL-6 and of pro-apoptotic proteins like cytochrome c, Bax, Bad, Apaf, caspase-3 and -9 revealed that Sec cor 30 suitably modulated their expression levels along with amelioration of skin papilloma. FACS data also suggested an increase of cell population at S and G2 phases and decrease in sub- G1 and G1 phages in carcinogen-treated drug-unfed mice, but these were found to be near normal in the Sec cor 30-fed mice. There was reduction in genotoxic and DNA damages in bone marrow cells of Sec Cor 30-fed mice, as revealed from cytogenetic and Comet assays. Changes in histological features of skin papilloma were noted. Immunofluorescence studies of AhR and PCNA also suggested reduced expression of these proteins in Sec cor 30-fed mice, thereby showing its anti-cancer potentials against skin papilloma. Furthermore, this study also supports the hypothesis that potentized homeopathic drugs act at gene regulatory level.
In vitro and in vivo anticancer properties of a Calcarea carbonica derivative complex (M8) treatment in a murine melanoma model.
Laboratório de Pesquisa em Células Inflamatórias e Neoplásicas Depto de Biologia Celular, Setor de Ciências Biológicas, Federal University of Paraná, Brazil.
Melanoma is the most aggressive form of skin cancer and the most rapidly expanding cancer in terms of worldwide incidence. Chemotherapeutic approaches to treat melanoma have had only marginal success. Previous studies in mice demonstrated that a high diluted complex derived from Calcarea carbonica (M8) stimulated the tumoricidal response of activated lymphocytes against B16F10 melanoma cells in vitro.
Here we describe the in vitro inhibition of invasion and the in vivo anti-metastatic potential after M8 treatment by inhalation in the B16F10 lung metastasis model.
We found that M8 has at least two functions, acting as both an inhibitor of cancer cell adhesion and invasion and as a perlecan expression antagonist, which are strongly correlated with several metastatic, angiogenic and invasive factors in melanoma tumors.
The findings suggest that this medication is a promising non-toxic therapy candidate by improving the immune response against tumor cells or even induce direct dormancy in malignancies.
Homeopathy is one of the most popular forms of alternative medicines in the world, and its popularity is growing as more and more people discover it. Many famous people have used homeopathy successfully. They include: Mark Twain, Mahatama Gandhi, Paul McCartney, Cindy Crawford, Catherine Zeta-Jones, Tina Turner, Orlando Bloom, Toby Maguire, Pamela Anderson, Lisa Marie Presley and Jennifer Aniston.
The entire royal family uses homeopathy. Prince Charles especially is a very vocal advocate of homeopathy.
In one country (India), the equivalent number people the same as the whole of the entire population of the United States uses Homoeopathic Medicine from Homoeopathic Doctors.
South Vancouver Family Place and Access Natural Healing are partnering in this presentation about homeopathy for the whole family. The focus is on children, with tips on how to safely and effectively manage fevers, ear infections and other common childhood ailments.
Taught by Access Natural Healing’s Classical Homeopath, Elena Cecchetto DCH, CCH, RSHom (NA), this class will focus on the practical application of remedies in common ‘acute’ conditions.
We will cover homeopathic treatment for wounds, bruises, burns, cuts, dental treatment, injuries, fractures, head injuries, nosebleeds, shock, sprains and strains, sunstroke, coughs, colds, chest complaints, teething, colic and earaches. Open discussion in the class allows for feedback on remedy use.
Ask about Part 2 of our workshop series after you’ve experienced this first introduction.
Where: South Vancouver Family Place, 7710 Nanaimo Street
When: Tuesday, Feb 22 from 12:45 to 2:45
Who: For families with young children ages 0 to 5
What else: Free childcare (please register) and snack
How: To register please call the Family Place or talk to the staff on site. (604-325-5213)