Japanese Encephalitis

American Journal of Infectious Diseases 6 (2): 24-28, 2010 ISSN 1553-6203 © 2010 Science Publications
Decreased Intensity of Japanese Encephalitis Virus Infection in Chick Chorioallantoic Membrane Under Influence of Ultradiluted Belladonna Extract

1Bhaswati Bandyopadhyay, 2Satadal Das, 1Milan Sengupta, 3Chandan Saha, 4Kartick Chandra Das, 4Debabrata Sarkar and 5Chaturbhuj Nayak 1Department of Microbiology, Virology Unit, School of Tropical Medicine, Kolkata-700073, India 2Department of Pathology and Microbiology, D.N. De H. Medical College, West Bengal University of Health Sciences, Kolkata-700046, India 3Department of Clinical and Experimental Pharmacology, School of Tropical Medicine, Kolkata-700073, India 4Drug Proving Research Centre, CCRH, Government of India, Kolkata-700 046, India 5Department of AYUSH, Ministry of Health, CCRH, Government of India, JLN Anudandhan Bhawan, 61-65 Intitutional Area, Janakpuri, New Delhi 110058


Abstract: Problem statement: No specific antiviral therapy is currently available despite an emergence and resurgence of Japanese encephalitis in South-East Asian Countries. There are only few recent studies, which were aimed to treat Japanese encephalitis with newer drugs. There is thus a real need for study on antiviral agents that can reduce the toll of death and neurological sequelae resulting from infection with this virus. Approach: Optimum dilution of the JE virus was determined which could produce significant number of pocks on Chorioallantoic Membrane (CAM). Then ultradiluted belladonna preparations were used to see their inhibitory action on JE virus infection in CAM. Results: Ultradiluted belladonna showed significantly decreased pock count in CAM in comparison to JE virus control. Conclusion: Ultradiluted belladonna could inhibit JE virus infection in CAM, which may be mediated through glycosidase inhibitory role of calystegines present in belladonna.
Key words: Japanese Encephalitis (JE), Chorioallantoic Membrane (CAM), pock, belladonna



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